To create a primer based on a selected region of sequence:

  1. Open the Primer Design view.
  1. Select some or all of the sequence.
  1. Select Priming > Create Forward Primer to create the primer on the forward strand, or Priming > Create Reverse Primer to create the primer on the reverse strand.

A new primer feature is added automatically and is visible in all graphical views. The forward/reverse primer is also added as an row in the Primers view.