1. Use File > Open to open the file or SeqBuilder Project (.sbd) containing the sequence fragment you wish to clone.
  1. Use any of the following methods to select the sequence fragment to use as the insert:
  1. Choose Cloning > Clone Selected Fragment, or Cloning > Clone Selected Fragment From Opposite Strand, as needed. The latter command selects the opposite orientation of the sequence.

The Project window opens with the Clone tab active. The selection from Steps 1-2 is placed in the Inserts folder in the Project tab, as well as in the Fragments section near the bottom right of the Clone tab.

Proceed to Step 2: Specify the method and options.