To manually create a new primer pair from two existing primers (as an alternative to creating primer pairs automatically ):

  1. Select the primers you wish to pair using either of these methods:
    • From within the Primers view, click on the row of the first primer, and then Ctrl/Cmd+click on the row for the second primer.

    • From within the Primer Design view, click on the first primer feature, and then Shift+click on the second primer feature.
  1. Select Priming > Pair Selected Primers.

A new primer pair is created from the two selected primers, and becomes the active pair.