In the following tutorial, you’ll locate open reading frames (ORFs), create a feature at the location of a pair of ORFs, and then change the way the feature is displayed.

  1. Download and extract the tutorial data.
  1. Use File > Open to open Lasergene ‘x’ Data\Demo SeqBuilder Pro\Tn5w.sbd, where ‘x’ denotes the version of Lasergene.

The upper pane of the window displays the Linear view, while the lower pane shows the Features view.

  1. To locate ORFs, use the Translations and ORFs tool on the left and select ORFs > Top Frame 1.

  1. Hold down the Shift key and select each of the adjacent ORFs around position 3700.

In the header, check that the current Selection is “Forward 3649..3867, length = 219.”

  1. Select Features > New Feature. A feature appears in the Linear view as an orange-brown box.

  1. In the bottom pane of the screen, note the flashing cursor to the right of the word /note. Type ORFs of interest and press Enter/Return; this notation now appears as the label for the feature in the Linear view.

  1. To make this the permanent label for the feature, right-click on /note and choose Use As Feature Label.
  1. Near the top-right of the SeqBuilder Pro window, click on the Style tab to make the Style panel active. In the Style panel:

    1. Click the orange-brown box to the right of Line and pick any shade of purple from the color chooser. Press OK.
    2. Change the Line Weight to 2.
    3. Add a shadow by checking the box next to Shadow.
    4. Use the Shape drop-down menu to select Filled Arrow.
  1. (optional) If desired, move the green zoom slider ( ) to the right to zoom in on the feature.

The feature now appears as below.