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Try it! – Gateway cloning
Automated Virtual Cloning » Try it! – Gateway cloning
Gateway cloning is based upon the site-specific recombination of lambda bacteriophage, and is a two-part process. In the first part of the tutorial, you will create a Gateway entry clone. In the second part, you will use the entry clone as input for constructing the…
Try it! – MultiSite Gateway Pro cloning
Automated Virtual Cloning » Try it! – MultiSite Gateway Pro cloning
MultiSite Gateway Pro technology enhances the classic Gateway cloning protocol by allowing you to clone up to four regions at one time. In MultiSite Gateway Pro cloning, three additional types of recombination sites have been engineered to recombine uniquely between…
Try it! – PCR-directed restriction cloning
Automated Virtual Cloning » Try it! – PCR-directed restriction cloning
This tutorial demonstrates how to cut and clone a signal transduction gene into the pENTR/D-TOPO vector. If you have not yet download and extracted the tutorial data, click here to download it. Then decompress (unzip) the file archive using the method of your…
Try it! – Create and modify a feature
Features » Try it! – Create and modify a feature
In the following tutorial, you’ll locate open reading frames (ORFs), create a feature at the location of a pair of ORFs, and then change the way the feature is displayed. If you have not yet download and extracted the tutorial data, click here to download it.…
Try it! – Gibson cloning with a custom vector
Automated Virtual Cloning » Try it! – Gibson cloning with a custom vector
The Vectors drop-down menu in the Project window is a shortcut to all of the vectors contained in the CloningVectors.sbp project, also known as the “Vector Catalog.” It is possible to add a custom vector to an open project, or to add it to the Vector Catalog…
Try it! – Use a PCR insert in TA cloning
Automated Virtual Cloning » Try it! – Use a PCR insert in TA cloning
The tutorial Try it! – Create and modify primers for TA cloning demonstrated how to select and modify a primer pair. This tutorial shows how to extract the PCR insert and create a virtual TA clone. If you have not already done so, follow the tutorial Try it!…
Try it! – Create a style set for CDS features
Plasmid Auto-Annotation » Specify the appearance of features in the Feature Library Manager and Annotation Results window » Try it! – Create a style set for CDS features
The Feature Library Manager can be used to specify how to display particular features or groups of features when auto-annotating plasmids/vectors in the Annotation Results window. This tutorial shows how to specify a particular style for displaying all CDS…
Try it! – Open an Entrez sequence by accession number
Open an Existing Sequence » Open a sequence from an online database » Open an Entrez database file by locus name or accession number » Try it! – Open an Entrez sequence by accession number
In this part of the tutorial, you will open a histone DNA sequence from NCBI’s Entrez database. In order to complete this tutorial, your computer must be connected to the Internet. Select File > Open Entrez Sequence. The Open Entrez dialog appears. Under…
Try it! – Apply a restriction enzyme to the sequence
Enzymes » Show or hide enzymes in other views » Try it! – Apply a restriction enzyme to the sequence
In this short tutorial, you’ll remove the restriction enzymes displayed on the sequence by default, and instead display only the DraI enzyme cut sites: If you have not already done so, follow the tutorial Try it! – Create and modify primers for TA…
Try it! – Create and modify primers for TA cloning
Primers » Edit primers » Introduce a codon change or mutation » Try it! – Create and modify primers for TA cloning
To create a primer pair for a region of interest on the sequence to use in TA cloning: If you have not yet download and extracted the tutorial data, click here to download it. Then decompress (unzip) the file archive using the method of your choice. Use File >…
Try it! – Find restriction sites on either side of a feature
Enzymes » Create and manage sets of enzymes » Try it! – Find restriction sites on either side of a feature
In this tutorial, you will look for a single enzyme capable of excising a histone coding feature from a sequence named X05543.1. To open the sequence, follow the tutorial Try it! – Open an Entrez sequence by accession number. You’ll need an enzyme that…
Features
Features
Features are annotations made to a specified range on a sequence. They can be imported as part of a sequence file (e.g., Genbank, EMBL, etc.), or they can be created within SeqBuilder Pro. See the following topics to learn how to: Create or delete features Edit…
Step 5: Run the cloning simulation
Automated Virtual Cloning » Step 5: Run the cloning simulation
When you have specified the insert and vector, and set up additional options as desired, you are ready to begin the cloning simulation: Press the Try It or Clone button in the bottom right corner of the Project window. The view switches from the Clone tab to the…
Specify the appearance of features in the Feature Library Manager and Annotation Results window
Plasmid Auto-Annotation » Specify the appearance of features in the Feature Library Manager and Annotation Results window
You can use the tools at the top of the Feature Library Manager to control how features are displayed in the Manager, itself, and in the Annotation Results window after performing the auto-annotation procedure. This step is entirely optional. For example, you could…
Automated Batch Cloning
Automated Batch Cloning
Automated batch cloning lets you automatically clone large numbers of fragments into the same vector. Most of the steps involved are similar or identical to those in regular automated virtual cloning, but the cloning window has been optimized for batch…
Site-Directed Mutagenesis
Site-Directed Mutagenesis
Site-directed mutagenesis is a molecular biology method that is used to make specific changes to the DNA sequence of a gene. As of Lasergene 16.0, you can perform site-directed mutagenesis using Protean 3D’s Protein Design service together with SeqBuilder Pro.…